Abstract. Isolates of Leptospira from two human cases of leptospirosis in Denmark and France were studied using DNA-DNA relatedness, G+C content, 16SrRNA gene sequence data and pulsed-field gel electrophoresis. These isolates differed from previously described species of Leptospira and are defined as Leptospira broomii sp. nov. The type strain is 5399T (=ATCC BAA-1107T =KIT 5399)
Comment. This paper does not provide serological information. However, since the isolates have been identified before as L. fainei serovar Hurstbridge (Petersen et al. 2001; Arzouni et al. (2002) Emerg Infect Dis 8, 865-868), strain 5399 has been assigned to serovar Hurstbridge in this Leptospira Library. It should be noted that the origin of strain 5399 is unclear. Information at the various authors confirm that this is an isolate from a Danish patient. However, it is unclear whether the strain 5399 is the same as strain SSI 5402-98 with accession number Y19243 as described by Peterson et al. One of the personal communications indicates that 5399 equals an isolate from a patient provisionally denoted as HB6, which in turn is the same as SSI 5402-98. For this reason we have chosen to denote the strain as a type of Hurstbridge, i.e. serovar Hurstbridge type HB6
* Levett PN, Morey RE, Galloway RL. Leptospira broomii sp. nov., isolated from humans with leptospirosis. Int J Sys Evol Microbiol (2008), 56, 671-673
* Petersen AM, Boyde K, Blom J, Schliting P, Krogfelt KA. First isolation of Leptospira fainei serovar hurstbridge from two human patients with Weil's syndrome. J Med Microbiol (2001), 50, 96-100
Conventionally, Leptospira were separated into two valid species, Leptospira interrogans for pathogenic leptospires and Leptospira biflexa for saprophytic ones (Brenner et al., 1999).
Current Leptospira speciation is based on DNA heterogeneity as determined by hybridization experiments.
The first of such studies was by Yasuda et al., 1987 followed by Ramadass et al., 1992.
The most complete study, also summarizing the observation of the previous two studies is by Brenner et al., 1999. Brenner and co-workers described 17 DNA hybridization groups (species), representing the 12 previously describes species and five new ‘genomospecies’, including Leptospira alexanderi.
Smythe et al., 2012 re-named the four remaining ‘genomospecies’ 1, 3, 4 and 5 later on to Leptospira alstonii, L. vanthielii , L. terpstrae and L. yanagawae, respectively.
To date, speciation is increasingly based on other molecular techniques, mainly DNA sequences of the rrs gene or multilocus sequence genotyping, which likely will completely replace the DNA hybridization method.
* Yasuda PH, Steigerwalt AG, Sulzer KR, Kaufmann AF, Rogers F, Brenner DJ. Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospiroceae with proposals for seven Leptospira species. Int J Syst Bacteriol 1987, 37, 407-415
* Ramadass P, Jarvis BDW, Corner RJ, Penny D, Marshall RB. Genetic characterization of pathogenic Leptospira species by DNA hybridization. Int J Syst Bacteriol 1992, 42, 215-219
* Brenner DJ, Kaufmann AF, Sulzer KR, Steigerwalt AG, Rogers F, Weyant RS. Further determination of DNA relatedness between serogroups and serovars in the family Leptospiroceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies. Int J Syst Bacteriol 1999, 49, 839-858
* Smythe L, Adler B, Hartskeerl RA, Galloway RL, Turenne CY, Levett PN. Classification of Leptospira genomospecies 1, genomospecies 3, genomospecies 4 and genomospecies 5 as Leptospira alstonii sp. nov., Leptospira vanthielii sp. nov., Leptospira terpstrae sp. nov., Leptospira yanagawae sp. nov., respectively. Int J Syst Evol Microbiol 2012;
* Ahmed A, Grobusch MP, Klatser P, Hartskeerl RA. Molecular Approaches in the Detection and Characterization of Leptospira. J Bacteriol Parasitol 2012, 3:2 http://dx.doi.org/10.4172/2155-9597.1000133
* Adler, B., & de la Peña Moctezuma, A. (2010). Leptospira and leptospirosis. Veterinary microbiology 140, 287-296.
* Cerqueira, G.M. & Picardeau, M. (2009). A century of Leptospira strain typing. Infection, Genetics and Evolution 9, 760-768.