Abstract: A Leptospira strain (designated RIM 139) was isolated from the kidney of a house-mouse, Mus musculus, in Tras-os-Montes e Alto Douro region of northern Portugal. The isolate showed typical leptospiral motility and morphology under dark-field microscopy and was pathogenic for hamsters. Species determination was carried out on basis of PCR products generated by species-specific primers and by sequencing of the secY gene. Putative serogroup typing was performed using the microscopic agglutination test (MAT) with a panel of rabbit anti-Leptospira sera representative of the major pathogenic serogroups. Serovar identification was carried out by a combination of monoclonal antibodies and cross-agglutinin absorption test (CAAT). The novel nature of the strain was confirmed by restriction endonuclease analysis (REA). Results showed that RIM 139 represents a new serovar. The name Altodouro is proposed for this new serovar. Speciation findings, PCR analysis of the ompL1 gene and sequencing of the secY gene, indicated that it belonged to Leptospira kirschneri as did amplification with G1/G2 and B64-I/B64-II the primer sets.
* Paiva-Cardoso MD, Arent Z, Gilmore C, Hartskeerl R, Ellis WA. Altodouro, a new Leptospira serovar of the Pomona serogroup isolated from rodents in northern Portugal. Infect Genet Evol, 2013;13:211-217. On line. Doi:10.1016/j.meegid.2012.09.013
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